Your source for comprehensive tools, services, and insights for the latest Live Cell Imaging (LCI) research
Understanding biological processes as they happen, in real time and in living cells, is key to modern life sciences research. Whether you're tracking dynamic changes in the cytoskeleton, observing mitotic events, or conducting high-resolution multicolour imaging, live cell imaging enables you to bring your biology to life.
At Tebubio, we’ve curated a powerful selection of dyes, stains, vectors, and labelling kits from globally recognised suppliers delivering exceptional photostability, low toxicity, superresolution compatibility, and more. Backed by expert guidance and tailored services, we’re here to help you design and execute impactful live-cell experiments.
Live Cell Imaging Research Products
Visualise life in motion with precision-designed tools for real-time cellular imaging.
At Tebubio, we’ve partnered with world-leading innovators in fluorescence and microscopy to offer you a high-performance portfolio of live-cell compatible stains and imaging tools. Each product is carefully selected to meet the demanding requirements of modern live-cell imaging:
- Photostability for long-term acquisition and minimal signal loss
- High cell permeability for efficient staining without fixation
- Low cytotoxicity, preserving natural cell behaviour and viability
- Multicolour compatibility for complex staining strategies, including superresolution, animal models, and multicolour workflows
Whether you’re designing experiments or optimising staining, we’re here to support you and simplify your live cell imaging process.
1. Superresolution Imaging Dyes – New!
2. Multicolour Stains for Core Structures
3. Far-Red and NIR Imaging for Animal Models
4. BacMan Vectors for Long-Term Live Imaging
5. Custom Labelling for Your Proteins and Antibodies
1. Superresolution Imaging Dyes – New!
Thanks to our partnership with abberior, we now offer specialised dyes for STED and SIM microscopy.
These ultra-stable, bright stains are available in a wide range of colours for lysosomes, Golgi, nucleus, membranes, cytoskeleton (actin, tubulin), and more. These stains are also ideal for multicolour super-resolution imaging.
Among the newest additions, abberior LIVE 510 mito (cyan) enables precise labelling of mitochondria in living cells, while abberior LIVE RED tubulin offers robust and bright visualisation of the microtubule network, both optimised for STED resolution and low phototoxicity in live-cell contexts (Figure 1).
| Confocal | STED |
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Figure 1: This side-by-side comparison highlights the resolution power of STED microscopy versus traditional confocal imaging. Developed by Nobel laureate Stefan Hell, STED (Stimulated Emission Depletion) microscopy significantly sharpens image detail by overcoming the diffraction limit, enabling precise visualisation of subcellular structures in live cells. The difference in resolution is especially critical when examining fine structures such as cytoskeletal elements, vesicles, or organelles.
We also offer innovative tools for click-chemistry compatible labelling in live cells. In the example shown, abberior LIVE 550 click (cyan) labels TCO*A-incorporated actin via a bioorthogonal reaction, while abberior LIVE 610 tubulin (yellow) directly stains the microtubule network. This combination enables crisp, two-colour STED imaging of fine cytoskeletal details in living cells.
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Figure 2: Experience the precision of multicolour live-cell STED imaging. Actin structures (cyan) were selectively labelled using abberior LIVE 550 click via bioorthogonal chemistry, while microtubules (yellow) were visualised with the direct probe abberior LIVE 610 tubulin. Together, these cutting-edge stains reveal the cytoskeletal architecture in stunning resolution, ideal for dynamic, multiplexed analysis in living systems. |
These images demonstrate how abberior LIVE dyes make advanced live-cell imaging not only possible, but remarkably effective for high-resolution, multicolour applications.
2. Multicolour Stains for Core Structures
To visualise key cellular structures in real time, we offer a carefully selected range of well-characterised, cell-permeable fluorophores, including Spirochrome's SiR-Actin, SiR-Tubulin, and SiR-DNA.
These highly referenced dyes are specifically designed for live-cell compatibility, offering excellent photostability, low cytotoxicity, and strong signal intensity, making them ideal for time-lapse and high-resolution imaging applications.
- For more complex imaging setups, SPY dyes and second-colour variants, such as SiR700-Actin, enable efficient dual or triple labelling strategies (see Figure 6), allowing researchers to visualise multiple structures simultaneously within the same sample.
- This approach is particularly valuable for monitoring dynamic cellular events such as mitosis (Figure 7), axonal growth, or cytoskeletal rearrangements (Figure 8), offering high-resolution insights without disturbing cellular physiology, essential for long-term or kinetic studies.
| SiR-Actin | SiR-Tubulin | SiR-DNA | SiR-Tubulin & SiR700-Actin | SiR-Tubulin | SPY650-FastAct |
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| Figure 3: Drosophila hind gut stained with SiR-Actin. | Figure 4: Rat cortex neuro stained with SiR-tubulin. | Figure 5: MCF10A Spheroid stained with SiR-DNA. | Figure 6: Double staining with SiR-Tubulin and SiR700-Actin. | Figure 7: Movie of HeLa cell expressing mcherry-H2B (red) stained with SiR-Tubulin (green). | Figure 8: Movie of axonal growth of SPY650-FastAct™ stained chicken dorsal root ganglia neurons in vitro. |
3. Far-Red & NIR Imaging for Animal Models
For deep-tissue or in vivo imaging, selecting the right fluorophores is crucial to obtaining meaningful, high-contrast data. That’s why we offer a specialised range of stains emitting in the far-red to shortwave-infrared (SWIR) spectrum, ideal for penetrating biological tissues while minimising background noise.
Thanks to our collaboration with Proimaging, you can access high-performance dyes specifically developed for live animal and tissue imaging:
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CJ215 Tumour Stain (λem = 825 nm): optimised for tumour labelling in small animal models, offering excellent tissue penetration and signal clarity in deep imaging contexts (see Figure 9)
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LBL-Dye CellTag 717: a far-red general-purpose cell stain for visualising cell distribution and morphology in tissue
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LBL-Dye Mito 715 targets mitochondria specifically, enabling precise mapping of metabolic activity or mitochondrial dynamics in vivo
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Figure 9: Cancer tumour visualisation in a mouse with CJ215 Generic Tumour Stain. |
These dyes are particularly well suited for preclinical studies, longitudinal tracking, and real-time imaging in whole organisms. Their emission properties help reduce autofluorescence and improve imaging contrast, especially in complex or autofluorescent tissues. Whether you’re working with tumour models, organ-specific labelling, or biodistribution studies, these tools offer clarity where conventional dyes may fall short.
4. BacMan Vectors for Long-term Live Imaging
For extended and precise tracking of organelles, cellular structures, and dynamic morphology, explore Montana Molecular's Paintbrush vectors. These BacMam-based episomal constructs are designed to express fluorescently tagged proteins in living cells, enabling researchers to visualise and monitor biological processes over time, without genome integration.
Thanks to their high transduction efficiency and low cytotoxicity, they’re ideal for long-term live-cell imaging, especially when stable yet non-permanent expression is needed. Paintbrush vectors are available in multiple colours, allowing flexible labelling strategies, including double or multiplex staining.
They provide:
- Sustained expression of fluorescent fusion proteins without genomic modification
- Specific labelling of key organelles and subcellular structures (see Figure 10)
- Compatibility with multicolour imaging workflows
- Real-time monitoring of processes like cell morphogenesis, trafficking, or signal transduction (see Figure 11)
Browse our selection of green fluorescent vectors and red fluorescent vectors to tailor your experiments to your microscopy setup and biological targets.
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| Figure 10: The Montana Red Paintbrush is a new, improved red fluorescent protein that is 2x brighter than mCherry and produces bright fluorescence in under 18 hours. Targeting motifs are used to localise mNeonGreen or Montana Paintbrush (red fluorescent) proteins to various cellular domains. | Figure 11: Green MitoPaint (#P1010G) and Red Lamp2Paint (#P1030R). Expressed in HEK293s, this video gives an overview of the cellular target structures which can be labelled with this technology. |
5. Custom Labelling for Your Proteins and Antibodies
Need to label your proteins, antibodies, or nanobodies for live-cell imaging?
We offer a complete range of ready-to-use labelling kits compatible with abberior dyes, allowing you to create custom fluorescent probes tailored to your experimental needs.
For even greater flexibility, explore the functionalised SiR and SPY dyes from Spirochrome, available as reactive derivatives for direct conjugation. These tools are ideal for generating high-performance, cell-permeable labels across a wide range of applications.
For far-red and SWIR imaging requirements, Proimaging offers a functionalised dye with an emission maximum at 832 nm, specifically designed for deep-tissue and in vivo imaging workflows.
To help you explore these possibilities in more detail, we’ve compiled several scientific resources and product highlights:
These documents offer technical depth and practical guidance, supporting you in building custom, reliable imaging tools with confidence.
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Live Cell Imaging Research Services
Your live-cell imaging projects are essential to revealing dynamic biological processes, but we understand they can come with technical complexity. At Tebubio, we do more than supply reagents. We provide dedicated scientific services designed to support and accelerate your imaging research, from initial concept to data acquisition.
Whether you’re fine-tuning a challenging staining protocol, developing a custom multicolour panel, or setting up live-compatible assays for high-content screening, our Contract Research Services (CRS) team is here to assist you, practically and proactively.
Need support with fluorophore-antibody conjugation? Looking for help designing a multiplex workflow tailored to your microscope and cell model? We work closely with you to develop fit-for-purpose solutions, aligned with your experimental goals, timelines, and imaging platforms.
With a team of PhD-level scientists and access to a network of trusted CRO partners, we help streamline even the most advanced workflows—so you can focus on discovery, not troubleshooting.
Explore our full imaging services to see how we can help bring greater clarity, flexibility, and confidence to your live cell imaging research.
Discover our Services
Looking for a reliable partner to design advanced live-cell imaging assays or refine your multiplex labelling strategies? Need expert advice to select compatible dyes or optimise staining conditions for dynamic cellular models? Our CRS team, based in France, provides bespoke support to help you streamline your imaging workflows and maximise the quality of your data.
With a team of dedicated scientists, including four experienced project managers, we offer end-to-end support tailored to your specific research goals and technical challenges, whether you're working with super-resolution microscopy, time-lapse imaging, or live-tissue applications.
Our laboratories are equipped with state-of-the-art platforms to support the full spectrum of Live Cell Imaging needs, including:
- Custom Imaging Assay Development: Live-cell compatible I Multicolour labelling I Superresolution
- Fluorophore Conjugation Services: Antibodies I Nanobodies I Proteins
- Staining & Protocol Optimisation: Low-toxicity reagents I Imaging buffers I Incubation conditions
- Dynamic Imaging Support: Time-lapse I Real-time visualisation I Cell tracking
- Data Analysis & Interpretation: Quantification I Custom Reporting
Let’s collaborate to simplify your live-cell imaging process, from reagent selection to data-ready images, so you can focus on advancing your science.
Explore our CRS Scientific Cases
Development and Imaging Validation of Fluorescent Lipid Nanoparticles for RNA Delivery Applicable to Personalized In Vitro Oncology Models
Discover how Tebubio's innovative LNP platform enables real-time tracking of RNA delivery in oncology models. Explore our latest findings and applications for personalized medicine.
Production of eGFP mRNA Labeled with Fluorophore for Tracking of Delivery and Expression
Our Contract Research Services (CRS) team developed eGFP mRNA labeled with a fluorophore to monitor its delivery and expression efficiency in cells
Our CRS Platforms at a Glance
Live Cell Imaging Knowledge Resources
Live Cell Imaging is advancing rapidly, with new technologies and methodologies constantly pushing the boundaries of what we can see and quantify. Staying informed is essential to making the most of these innovations.
Whether you're searching for expert tips, technical protocols, or the latest developments in imaging techniques, Tebubio’s Knowledge Hub offers a rich collection of resources tailored for imaging researchers. Designed to help you stay at the forefront of your field, our content combines practical tools with scientific insights.
Explore our library of articles, application notes, white papers, and webinars covering key topics such as live-cell imaging, multiplex fluorescence, spatial biology, and advanced image analysis. Gain new perspectives, learn how to optimise your experiments, and sharpen your imaging strategies with guidance from trusted experts.
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References
Figures 1 & 2: Courtesy of abberior, your trusted supplier for superresolution microscopy tools.
Figures 4 to 8: Courtesy of Spirochrome, your trusted supplier for live cell imaging dyes.
Figure 7: Movie courtesy of Daniel Gerlich and Claudia Blaukopf, Institute of Molecular Biotechnology, Vienna.
Figure 8: Movie courtesy of Alexandre Dumoulin, University of Zürich, Switzerland.
Figure 9: Courtesy of Proimaging, your trusted supplier for tumour stain.
Figures 10 & 11: Courtesy of Montana Molecular, your trusted supplier for BacMan Vectors.
Ready to take your Live Cell Imaging further?
Please contact us for further information about our live cell imaging solutions. Our team is available to provide detailed insights tailored to your research needs.
