Extend Protein Expression and Duration with ModTail™ mRNA from TriLink

A Smarter Way to Optimise mRNA Performance

 

Developed by TriLink BioTechnologies, the ModTail™ poly(A) tail modification is a proprietary innovation designed to enhance the performance of synthetic mRNA. By introducing a small chemical moiety to the 3′ end of the poly(A) tail after in vitro transcription (IVT), ModTail™ extends protein expression and duration without altering the coding sequence or compromising integrity.

Compatible with standard delivery systems such as lipid nanoparticles (LNPs) and transfection reagents, ModTail™ offers a seamless upgrade to your current workflow.

 

 

Proven Performance

In both cell-based and in vivo models, ModTail™ mRNA consistently increases and prolongs protein expression:

  • In HEK293T cells, ModTail™ mRNA led to higher and longer-lasting eGFP fluorescence compared to unmodified controls (Figure 1).

  • In mice, ModTail™ mRNA delivered via LNPs produced stronger luminescent signals at later timepoints, demonstrating extended protein expression in vivo (Figure 2).

These results confirm ModTail™ as a simple yet powerful enhancement to mRNA performance.

Figure 1: ModTail mRNA enhances protein expression and persistence in HEK293T cells
In HEK293T cells, eGFP mRNA, either modified with TriLink’s ModTail™ poly(A) tail or unmodified, was encoded and transfected using CleanCap® M6-capped mRNA. eGFP protein fluorescence was monitored over time using Incucyte®.

 

 

Figure 2: Mice injected with ModTail mRNA show increased duration of protein expression

Mice were injected with PBS (top rows) or Fluc mRNA: LNPs for whole body luminescent imaging at 12, 48, and 72 hours. For both CleanCap® AG 3’OMe (left panel) and CleanCap® M6 (right panel) capped Fluc ModTail mRNA (bottom rows) resulted in higher expression signal at later timepoints.

 

 

Ready-to-Use ModTail™ mRNAs


Choose from our optimised, high-expression constructs:

Each is formulated for robust and sustained protein production.

Custom ModTail™ Services

 

TriLink’s proprietary ModTail™ poly(A) tail modification is available through their custom mRNA services. This modification reliably enhances protein expression and prolongs protein duration in both cell-based assays and in vivo mouse studies. Let us manage your project from start to finish.

Contact us to initiate your next custom ModTail™ mRNA project!

 

Key Features

  • Mimics mature mRNA for efficient translation
  • CleanCap® M6 capping for ≥30% higher expression
  • Poly(A) tail enhanced with TriLink’s proprietary ModTail™ modification
  • Incorporates N1-methylpseudouridines for reduced immunogenicity

Applications

Ideal for:

          • Gene editing (e.g., Cas9)
          • Reporter assays (e.g., EGFP, FLuc)
          • Therapeutic protein delivery
          • Vaccine development

 

FAQ

Find answers to the questions most frequently asked by our users, curated by our experts.

  • What is TriLink’s poly(A) tail modification?

    TriLink’s proprietary poly(A) modification is a small chemical moiety linked to the 3’-end of the poly(A) tail of mRNA after the in vitro transcription reaction. There are no other changes to the mRNA.

  • Do I need to change my coding sequence or DNA template to use TriLink’s poly(A) tail modification?

    No, the modification is not template-directed and occurs post-IVT. In this case, there is no need to change your DNA template, and there is no impact on the mRNA coding sequence.

  • Have you tested the tail modification with different poly(A) tail lengths and with different mRNA constructs of varying lengths?

    Yes, we have tested our proprietary poly(A) tail modification with different poly(A) tail lengths including 40A, 60A, 80A, 100A, and 120A. We have successfully added our Poly(A) tail modification to various mRNAs, such as eGFP, Fluc, mCherry, beta-gal, and Cas9 mRNA. These constructs range from 0.9 to 4.5 kb in length.

  • Do I need to change how I deliver the mRNA?

    TriLink’s poly(A) tail modification should be compatible with commonly used delivery vehicles. We have used it with common transfection reagents and LNPs, including LipofectamineTM MessengerMAXTM reagents, and ALC-0315, DSPC, Cholesterol, and DMG-PEG 2000: Pfizer/Acuitas Lipids.

  • Does the modification increase the immunogenicity or toxicity of the mRNA?

    We have not observed meaningful differences in the immunogenicity of tail-modified mRNA from non-tail-modified mRNA in our in vitro and in vivo studies.

  • Does the modification impact the integrity of my mRNA?

    No, we have data showing that the mRNA is of comparable integrity to regular oligo-dT-purified mRNA.

Tebubio: Your European Partner for mRNA Research

As TriLink’s official EU distributor, Tebubio offers more than access: we provide expert support and local convenience:

  • Scientific Expertise: Our team understands IVT and mRNA synthesis at every level. Need help designing the right protocol, selecting the optimal reagents, or troubleshooting yields? We’re here to advise.
  • Faster Delivery: Order directly through our webshop with fast delivery across Europe.
  • One-on-One Support: Talk directly to scientists, not just sales. We help researchers every day make informed product decisions.
  • Regulatory Insight: Whether you’re working on RUO, preclinical, or GMP-grade transitions, we help navigate the right path.

Agnès Marcilly, Digital Campaign Specialist

Marketing Team at Tebubio

"ModTail™ mRNA redefines performance at the molecular level — delivering longer expression, greater stability, and seamless integration into your research and therapeutic workflows."

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Ready to Elevate Your mRNA Workflow?
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  • References

    Article content created by Tebubio using courtesy materials provided by TriLink Biotechnologies.

Knowledge Resources – Expand Your Expertise

Stay at the forefront of mRNA research with Tebubio’s latest insights about TriLink’s products. Read our blogs:

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