Page 10 - Scientific Library
Cell Signalling - how is the Phospho Explorer antibody array used?
If your research interest is focused on the identification of cell signalling pathways and protein phosphorylation profiles, the comprehensive Phospho Explorer Array may be of great help to you, measuring
Profile 2000 protein targets with the Human L2000 antibody array
RayBiotech , the antibody array specialist, have just released a new, very broad antibody microarray, the L2000 series, for the profiling of 2000 protein targets including metabolic enzymes, structural
Luciferase promoter reporter clones
Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient
Achieve success with RNA-based CRISPR-CAS9 knock-out
RNA-based CRISPR-CAS9 gene editing is a vector-free approach that is required for therapeutic perspectives. However, from a practical point of view, what appears as a major challenge to engineer primary
How to produce high yield capped mRNA
Messenger RNA can be used for protein expression into the cells. The delivery of mRNA is the delivery of a new function that can activate a biological process. In the particular case of the CAS9 mRNA,
Gold standard one component TMB
Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to blue reveals the activity
How to obtain reliable DNA and RNA quantitation
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in vitro
How to get rid of fibroblastic contaminating cells?
Tissue culture for Primary cell isolation are often jeopardized by the outgrowth of contaminating fibroblasts. As fibroblasts usually grow at a much faster rate compared to the target cell population,
Lympholyte®: The ideal tool for simple and viable lymphocytes isolation
High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE® have developed an efficient
Screen 500 mouse proteins on a single array
In a previous post, I introduced the G-Series Mouse antibody arrays to profile up to 200 mouse proteins using a combination of 5 antibody arrays on glass slide. This new Mouse L2 L-Series (label based
Hepatic Hyaluronan Content increased by Alcohol Consumption in Patients with Liver Steatosis
In a previous post, I introduced Sekisui Xenotech's Research Biobank, which is based on rejections from liver transplantation as opposed to post-mortem tissues, thus offering a guarantee for better tissue
Proteomics Biostats: get the most out of your array data
High density antibody arrays represent powerful technologies which have opened a new era in functional proteomics. However, when faced with a large magnitude of array data, you must apply comprehensive