Ac-Tyr-Val-Ala-Asp-AMC (Caspase 1 (ICE) Substrate) - 25 mg

CAS Number: 149231-65-2Molecular Weight: 665.27Salt Form: N/APurity: >96%Sequence (3-letter): Ac-Tyr-Val-Ala-Asp-AMCSequence (1-letter): Ac-YVAD-AMCStorage: -20 °C or belowCaspase 1 (ICE) Substrate (Ac-Tyr-Val-Ala-Asp-AMC) is a fluorogenic substrate for interleukin 1b converting enzyme (ICE) which is a cysteine protease that cleaves specifically at aspartic acid. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein which is a potent inhibitor of apoptosis inhibits activation of these proteases in BRK cells.Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.