RNA Labeling Kit – 647 - 1 Kit

The RNA Labeling Kit produces alkyne-modified RNA by in vitro transcription from bacteriophage T7 RNA polymerase promoters. The kit utilizes 5-Ethynyl-UTP (5-EUTP) nucleotide to firstly incorporate alkynes into RNA. The resulting RNA contains alkyne modified Uracil, which subsequently react with azides of e.g. fluorescent dyes, haptenes and others in a highly selective fashion under benign click reaction conditions. One advantage is that the label incorporation can be tuned by the UTP to 5-EUTP ratio.