Why dsRNA Removal Matters
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Double-stranded RNA (dsRNA) is a major immunogenic by-product of the in vitro transcription (IVT) process, caused by aberrant T7 RNA polymerase activity. Even at trace levels, dsRNA contaminants can:
For researchers developing mRNA therapeutics, dsRNA is a critical quality attribute. Eliminating it ensures higher purity, improved translation, and safer outcomes in applications such as cell and gene therapy and mRNA vaccines. |
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The Min-Immune™ Gold Solution
Tebubio brings you the Min-Immune™ Gold dsRNA Removal Kit, designed to simplify and accelerate dsRNA removal—without the need for expensive, labor-intensive HPLC methods.
Key Benefits at a Glance
- Easy, scalable enzymatic dsRNA removal from IVT RNA samples
- Ultra-low dsRNA levels (<0.005% LLOQ) to minimize immunogenicity
- Preserves ssRNA yields for reliable downstream use
- Compatible with modified nucleoside transcription for maximum reduction of immunogenicity
- Each kit (25 reactions) treats 1.5 mg of IVT RNA or mRNA
Proven Performance
Case Study: Comparison between untreated and Min-Immune Gold-treated samples
A 1.4 kb pseudouridine-containing RNA sample treated with Min-Immune™ Gold showed dsRNA reduction to below quantifiable levels (<0.005%), as demonstrated by immunoblot analysis (Figure 1).
Figure 1: Immunoblot of Min-Immune™ Gold dsRNA Removal Kit treated RNA. dsRNA is reduced below the lower limit of quantification after treatment.
Build a Complete Low-Immunogenicity Workflow
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For optimal results, combine the Min-Immune™ Gold Kit with modified nucleosides available through Tebubio:
This workflow delivers maximum reduction of immunogenicity for next-generation therapeutics, vaccines, and cell-based research. |
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References
Images: Coutesy of CellScript our trusted partner.
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