Scientific Library
Sircol for soluble and insoluble collagen assays!
Measuring soluble collagen in biological samples is a straightforward experiment which can be done with the Sircol soluble collagen assay using Sirius Red dye (Sircol dye) in a convenient manner (we explored
AAV - advantages for Gene delivery in vivo
Adenoassociated viruses (AAV) are small (20-30 nm), ssDNA, non-enveloped and replication-defective viruses that infect human and other primate species, but which, up to now, have not been reported as being
Adenovirus: weapons of mass transduction for gene delivery
Adenovirus (AV) are non-enveloped and non-integrative icosaedral viruses. Recombinant AV (genetically modified with respect to the wild-type in order to lose their capacity of replication) are widely used
HD-LCI video of HUVEC Transfection with Cytofect Kit
See human cells glow with green fluorescent protein in this time-lapse video. These images were captured over 20 hours via Lumascope 620.
[youtube https://www.youtube.com/watch?v=k5esUe_rrr4]
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High Definition
How to measure Glycosaminoglycans and Proteoglycans?
Glycosaminoglycans (or mucopolysaccharides) are long un-branched polysaccherides which consist of disaccharide repeats. The repeats usually (with the exception of Keratan sulfate) consist of on amino sugar
A simple way to measure Elastin
Recently I reported about methods to measure:
- Collagen - Sircol for soluble and insoluble collagen assays
- Glycosaminoglycans - How to measure Glycosaminoglycans and Proteoglycans?
Today, I invite you to
3 easy colorimetric assays to measure ECM components
Matrix components are key R&D elements in pharmaceutical and cosmetology with tremendous impacts on tissue regeneration, skin repair, wound healing, allergy
Cost-efficient pre-optimized transfection reagents
The main challenge when choosing a transfection reagent is that we don't know how it will work with our own cell type of interest. It is also time consuming to find the optimal conditions. Well, here's
Soluble and Insoluble Collagen assays - made easy!
The monitoring of ECM components during experimental approaches requires robust and user-friendly in vitro assays. In the past experimental methods (e.g. soluble collagen measurement) were laborious
Quantify ExtraCellular Matrix components - 5 popular kits you need to know
Immunoassays with collagen: Tips to optimize anti-collagen antibody performance
Dr Yong Zhang, Ph.D. is a senior scientist and R&D Manager for antibody generation and assay development at Rockland Immunochemicals, Inc. who are recognised and renowned for their high quality primary