Scientific Library
Pluripotent stem cells for improved reprogrammed Human Sebocytes
Following the start of our recent collaboration with Phenocell, we’re pleased to be able to provide high quality Sebocytes developed from Human
How to optimise your recombinant protein purification and protein production
Undoubtedly, producing and purifying a protein is an art form.
Indeed, each recombinant protein (rec. protein) to be produced in vitro
ELISA test to confirm elimination of Host Cell Proteins (HCP) from bioproductions
Expression systems - removing their contaminants
CHO, E. Coli and Pichia are useful expression systems for drug production. This can be for proteins, enzymes or antibody productions. Research and
Bioactive neuronal lipid mediators: a focus on Synaptamide
Focus Biomolecules produces a broad range of bioactive lipid mediators as well as many small molecule inhibitors of numerous lipid metabolic pathways for use as cellular
Tips and Tricks - Protein purification and production for LC-MS characterization
Mass spectrometry is a technology which is widely used in most scientific disciplines that require accurate and precise measurement of elemental and molecular components. Its use in the pharmaceutical
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily
Neurons, Astrocytes, Microglia: High Quality primary cells source
In the field of neurosciences, increasing research is being undertaken on neurodegenerative disorders like Parkinson’s and Alzheimer’s diseases, as well as on nerve injury and regeneration. One of the
Tips and Tricks: how to increase unstable protein expression in E. Coli
Recombinant protein expression and purification for therapeutic development or for in vitro studies can sometimes be a real challenge. It requires huge investments in both time
Why you should outsource your production of proteins - both stable or unstable
Whether your protein is simple to produce, or complex and requires more development, in any case, outsourcing your protein expression, purification and production is a very viable option
Consistent, scalable human iPSC-derived glutamatergic neurons and skeletal myocytes
As detailed in a separate post, bit.bio have developed a novel cellular reprogramming technology, opti-ox™
ELISA : Quality Control and Performance Evaluation
The ELISA test (acronym of Enzyme Linked ImmunoSorbent Assay) is a test principally used In immunology to detect and/or assay a protein, antigen or antibody in a biological fluid sample . It has many practical
Mitochondria-Lysosome Contacts in Neurogenetic Diseases
Mitochondrial Dynamics and Mitochondria-Lysosome Contacts in Neurogenetic Diseases
In an article published in frontiers in Neuroscience, with the aim to study cellular phenotypes