Page 2 - Scientific Library
Simple and effective CRISPR CAS9 gene editing for primary cells
Vector-free CRISPR-CAS9 gene editing to accelerate therapeutic applications
A few years ago, Ayal Hendel et al (doi:10.1038/nbt.3290) published results revealing that chemical alterations to sgRNA
Develop robust and convenient cell based assays with Gaussian Luciferase
Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is
Luciferase promoter reporter clones
Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient
Achieve success with RNA-based CRISPR-CAS9 knock-out
RNA-based CRISPR-CAS9 gene editing is a vector-free approach that is required for therapeutic perspectives. However, from a practical point of view, what appears as a major challenge to engineer
How to produce high yield capped mRNA
Messenger RNA can be used for protein expression into the cells. The delivery of mRNA is the delivery of a new function that can activate a biological process. In the particular case of the CAS9 mRNA,
How to obtain reliable DNA and RNA quantitation
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in
New - you know CRISPR, now it's Prime Editing
This month, a new leap forward in gene editing has been published in Nature under the title 'Search-and-replace genome editing without double-strand breaks or donor DNA'. DOI: 10.1038/s41586-019-1711-4.
What is the connection between COVID-19 severity and gene expression in immune cells?
The immune system, responsible for fighting off infections and diseases, refers to multiple biological processes that have different functions and contributions to the immune system and its response to
What diseases are linked to Microbiota disturbance?
The microbiota is the set of microbial organisms in a community, generally used in reference to an animal host. The digestive microbiota is the most common.
What we call a microbiome is the set
How can CRISPR/Cas9 be used to edit genomes?
Genes are key components of our lives. With many other factors, they contribute to shaping us, making people and any other living organism what they are. Genomes, whether human or not, are able