CheKine™ Micro Proline Dehydrogenase (ProDH) Activity Assay - 96 T

Proline dehydrogenase (ProDH) is a key enzyme that catalyzes the degradation of proline in mitochondria. Reducing the activity of ProDH is of great significance for regulating osmotic balance, preventing plant damage caused by osmotic stress, scavenging free radicals and protecting cell structure. CheKineTM Micro Proline Dehydrogenase (ProDH) Activity Assay Kit can be used to detect biological samples such as animal and plant tissues,cells or bacteria other liquid samples. In this kit, ProDH catalyzed the dehydrogenation of proline to pyruvic acid, and the removed hydrogen was transferred to reduce 2-dichlorophenol indophenol (DCPIP) by phenazine dimethyl ester sulfuric acid (PMS), and there was a characteristic absorption peak at 600 nm. Through the decrease of 600 nm absorbance, the reduction rate of 2-dichlorophenol indophenol (ProDH) was measured, which represented the activity of DCPIP.