Scientific Library

Matrix components are key R&D elements in pharmaceutical and cosmetology with tremendous impacts on tissue regeneration, skin repair,

Three papers on the role of the TGF-beta pathway in different cancers have recently been published.

It was already known that this pathway is involved in processes such as cell growth,

The Wnt cell signalling pathway is a high-potential therapeutical target in cancer research. It's deeply linked to cellular fate through modulating cell proliferation, mobility, interaction, and polarity.

Hyaluronic acid (Hyaluronan) belongs to the group of glycosaminoglycans (GAGs), but unlike other GAGs like Heparan sulfate

The monitoring of ECM components during experimental approaches requires robust and user-friendly in vitro assays. In the past experimental methods (e.g. soluble collagen measurement) were laborious

Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is

In living organisms, ExtraCellular Matrix (ECM)

Dr Yong Zhang, Ph.D. is a senior scientist and R&D Manager for antibody generation and assay development at Rockland Immunochemicals, Inc. who are recognised and renowned for their high quality primary

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target