Scientific Library

The Wnt cell signalling pathway is a high-potential therapeutical target in cancer research. It's deeply linked to cellular fate through modulating cell proliferation, mobility, interaction, and polarity.

Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade

The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results. Several factors can cause false-positive or false-negative

Generating high-quality Immunoprecipitation & Western Blot (IP & WB) data has never been easier. See how you can easily achieve this in our Tips & Tricks section to get clean WB-IP.

The TrueBlot® reagents

Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we'll review the main biomarkers involved in this disease and introduce the most popular validated

Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is Firefly

My colleague Ana introduced in a previous post (3 years ago already!) a selection of the most popular anti-tag antibodies among the research community. I must say that they remain extremely popular, showing

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of

We understand how crucial it is to have access to sustainable, quality sources of reagents when developing and/or producing the same immunoassays over long periods of times. Over the years, we've established