Scientific Library

A recent review by Nadim et al. casts some light on a Cosmetology and Dermatology, where circulating biomarkers, though studied to a certain extent so far, are yet unknown for many skin models.

Three papers on the role of the TGF-beta pathway in different cancers have recently been published.

It was already known that this pathway is involved in processes such as cell growth,

The Wnt cell signalling pathway is a high-potential therapeutical target in cancer research. It's deeply linked to cellular fate through modulating cell proliferation, mobility, interaction, and polarity.

Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is

Enteroviruses are common human viruses causing rather mild symptoms. However many different species and serotypes (incl. Echoviruses, Polioviruses,

Released by the kidneys, Renin is an enzyme which belongs to the Renin-Angiotensin system (RAS) and is involved in blood pressure homeostasis.

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

Tissue culture for Primary cell isolation are often jeopardized by the outgrowth of contaminating fibroblasts. As fibroblasts usually grow at a much faster rate compared to the target

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

Tropical and vector-borne diseases account for more than 17% of all infectious diseases, causing more than 1 million deaths annually. Recognizing infected patients early in their clinical course is critical

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target