Page 5 - Scientific Library

CRISPR/Cas9 is relatively simple to implement, as the researcher fully controls the experimental design of the tools, from the sgRNA sequence to the Cas9 protein.

A recent article by E. Langer (E. Langer, “Another In-House Operation Gets Outsourced” (2015) Pharmaceutical Technology 39 (6)) describes the growing

Isolation of highly purified untagged proteins is crucial in today's R&D programs. Up to now, recombinant protein production approaches were often based on the use of "tags" to make protein purification

The role of STAT3 signaling in the activity of dendritic cells (DC) has been recently revealed in a publication by Okada et al.

Pharmaceutical, biotech and medical device industries apply restrictive quality systems in their bio-production processes. Among the numerous parameters controlled daily by scientists, I have selected

A publication earlier this year by Bárcena et al. shows that angiogenin plays a crucial role in the onset and development of liver cancer (hepatocellular carcinoma or HCC).

Angiogenin was the first

SUMO-tag gene fusion acts as both a chaperonin and as an initiator of protein folding in both prokaryotic and eukaryotic systems. Addition of this tag enables protein experts to increase the expression

In a previous post, we looked at the importance of sample preparation methods depending on the biomarker being studied (e.g. MMPs, cytokines). Today, let's focus on cell / tissue lysates.

Tip #1

Traditional methods to detect and purify poly-ubiquitinated proteins require either anti-ubiquitin antibodies or Ubiquitin Binding Associated domains (UBAs). Most of the current methods display rather

Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade

Stem Cells: a great tool for biomedical research! From the embryo at a very early stage of development, stem cells have two important capabilities: to multiply to infinity by simple division and to give

The main challenge when choosing a transfection reagent is that we don't know how it will work with our own cell type of interest. It is also time consuming to find the optimal conditions. Well, here's