Scientific Library

Expression systems - removing their contaminants

CHO, E. Coli and Pichia are useful expression systems for drug production. This can be for proteins, enzymes or antibody productions. Research and

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

Interestingly, the experimental design is simple and is based on 3 steps:

1. Plate
2. Treat and fix your cells of interest (adherent or in suspension)
3. Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)

These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily

As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras