Scientific Library
Tips and Tricks: how to increase unstable protein expression in E. Coli
Recombinant protein expression and purification for therapeutic development or for in vitro studies can sometimes be a real challenge. It requires huge investments in both time and cost in order to obtain
Why you should outsource your plasmid preps
Plasmid preps are to science what salt is to cooking. They're so common that nobody notices, but nothing can be done without them. There is no mention of this step in any published paper, nobody mentions
Why you should outsource your production of proteins - both stable or unstable
Whether your protein is simple to produce, or complex and requires more development, in any case, outsourcing your protein expression, purification and production is a very viable option for your projects.
ChIP for sensitive & fast genomic mapping
Chromatin profiling assays enable the mapping of protein-DNA interactions that influence chromatin modeling and gene expression.
Over the years, Chromatin ImmunoPrecipitation (ChIP) assays became the gold-standard
Consistent, scalable human iPSC-derived glutamatergic neurons and skeletal myocytes
As detailed in a separate post, bit.bio have developed a novel cellular reprogramming technology, opti-ox™1 (optimized inducible overexpression), that can generate functional cells with high consistency
Meet the new CUT&RUN compatible Antibodies
Developed by Epicypher and available in Europe through tebu-bio, CUTANA CUT&RUN and its sister technology CUT&TAG are quickly becoming the preferred approaches for Chromatin Mapping experiments over the
Tips and tricks to design and test qPCR primers - advanced procedure
Tips and tricks to design and test qPCR primers - advanced procedure
Design and test your own qPCR primers
- Find the NCBI Gene page for the gene you would like to study. For example, for Human GAPDH.
- Scroll
What is epigenetics?
Each of our cells contains our entire genetic heritage: 46 chromosomes inherited from our parents on which there are approximately 25,000 genes.
But if all our cells contain the same information, they
How do cells protect themselves from viruses?
More than two years ago, the whole planet got impacted by COVID-19. Over time, and with the help of our dedicated worldwide scientific research community, we have learnt how to prevent its spread and to
ELISA : Quality Control and Performance Evaluation
The ELISA test (acronym of Enzyme Linked ImmunoSorbent Assay) is a test principally used In immunology to detect and/or assay a protein, antigen or antibody in a biological fluid sample . It has many practical
What is the future of mRNA based therapeutics, and their main challenges?
mRNA is a ribonucleic acid used by cells as an intermediate carrier of the genetic information contained in the DNA to synthesize proteins, and corresponds to the copy of one of the two strands of DNA.
Mitochondria-Lysosome Contacts in Neurogenetic Diseases
Mitochondrial Dynamics and Mitochondria-Lysosome Contacts in Neurogenetic Diseases
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In an article published in frontiers in Neuroscience, with the aim to study cellular phenotypes in fibroblasts from