Page 3 - Scientific Library

Following on from my previous post  in which I started to introduce a series of techniques to investigate the impact of miRNA expression on cell fate,

In this third post of the miRNA-related series, let's take a look at miRNA overexpression.

miRNA mimics

Short RNAs are very prone to enzymatic degradation. Novel chemical technologies now

Following on in our series on microRNAs (miRNA - Keys to open cell biology’s secret chamber)

Leading on from my previous posts (see below) exploring microRNA detection, overexpression and inhibition, this time, let's spend a moment on target detection.

miTarget databases

The cellular and physiological effects resulting from hypoxia-dependent networks have been clearly shown to impact a number of human pathological states, including ischemic disease, diabetes, pulmonary

Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.

Basically, the technology allows you to start

As part of the innate immune response, Toll-like receptor 9 recognizes unmethylated CpG nucleotide sequences, which are very common in viruses and prokaryotes and more rare in vetebrates. Scientists routinely

Adenoassociated viruses (AAV) are small (20-30 nm), ssDNA, non-enveloped and replication-defective viruses that infect human and other primate species, but which, up to now, have not been reported as being

Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and

Extracellular vesicles in general, or exosomes in particular, are becoming a hot topic for research, especially in cancer. They seem to have different roles in tumour progression or can

Adenovirus (AV) are non-enveloped and non-integrative icosaedral viruses. Recombinant AV (genetically modified with respect to the wild-type in order to lose their capacity of replication) are widely used

Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing