Page 11 - Scientific Library

Following on in our series on microRNAs (miRNA - Keys to open cell biology’s secret chamber)

Human Newborn Foreskin Fibroblasts (NuFF cells) are genuine feeder cells for Stem cell research. Mitotically inactive NuFF cells (by irradiation or Mitomycin C chemical treatment) can

Leading on from my previous posts (see below) exploring microRNA detection, overexpression and inhibition, this time, let's spend a moment on target detection.

miTarget databases

The cellular and physiological effects resulting from hypoxia-dependent networks have been clearly shown to impact a number of human pathological states, including ischemic disease, diabetes, pulmonary

Several lines of evidence show that synthetic lethal interactions targeting DNA repair pathways may have clinical applications in cancer therapy. In a recent paper, Albarakati et al. demonstrate

In a previous post on Cellular Matrices for Translational Research, I introduced HyStem hydrogels.  In order to engineer

In vivo monitoring of tumor growth and metastasis provides a powerful means for studying cancer properties and development of effective therapies. Mouse models created with tumor xenografts, resulting

Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.

Basically, the technology allows you to start

As part of the innate immune response, Toll-like receptor 9 recognizes unmethylated CpG nucleotide sequences, which are very common in viruses and prokaryotes and more rare in vetebrates. Scientists routinely

In this paper (Role of extracellular calcium and mitochondrial oxygen species in psychosine-induced oligodendrocyte cell death), Voccoli et al. used Human oligodendrocyte MO3.13 cells to study Globoid cell leukodystrophy.

Globoid cell leukodystrophy (GLD) is a metabolic disease caused by mutations in the galactocerebrosidase (GALC) gene. GALC is a lysosomal enzyme whose function is to degrade galacto-lipids, including galactosyl-ceramide and galactosyl-sphingosine (psychosine, PSY). GALC loss of function causes progressive intracellular accumulation of PSY. It is widely held that PSY is the main trigger for the degeneration of myelinating cells and progressive white-matter loss. However, still little is known about the molecular mechanisms by which PSY imparts toxicity. In this paper, the role of calcium dynamics during PSY-induced cell death is addressed. Using the human oligodendrocyte cell line MO3.13, the authors report that cell death by PSY is accompanied by robust

During the last International Federation of Societies of Cosmetic Chemists (IFSCC) congress (Paris, Oct. 2014), Hajem N. et al (ALES Groupe) have shown that by enhancing MDR activity of fibroblasts

Assessing gene transcription as an endpoint for determining induction of drug metabolizing enzymes and transporters has become the “method of choice” in the FDA’s latest draft Guidance for Industry drug