Page 12 - Scientific Library
Using PBMCs in immune cell studies
The availability of human peripheral blood mononuclear cells (PBMC) from healthy individuals and from patients with various diseases allows for many studies on normal and abnormal functions of human immune
Activation & de-activation pathways of hepatic fibrosis
3 fluorogenic substrates for iPS and Stem cells analysis
Methods commonly used for the characterization of iPS or ES cells are based on specific antibody staining. Nevertheless, undifferentiated stem cell populations can also be characterized by high levels
What's Trending in the Molecular Biology World? CRISPR !!!
It's official, CRISPR has officially reached "Breakout" status as a Google search term (see image from Google Trends). The Clustered, Regularly Interspaced, Short Palindromic Repeats (CRISPR)Â
6 tips for thawing hepatocytes
Cryopreserved hepatocytes contain the major hepatic drug-metabolizing enzymes required to study the four categories of xenobiotic biotransformation: hydrolysis, reduction, oxidation and conjugation. Cryopreserved
Proteome, secretome, kinome… What’s it all about?
Proteome is referenced as the result of expression of the whole genome. With the recent developments of genomics, one could consider that this analysis is enough to decipher any biological mechanism...
Distinctions between homogeneous bulk cancer cell tumors vs. Cancer Stem cell driven tumors by Pr. Clarke
Professor Alan Clarke, (Director of the European Cancer Stem Cell Research Institute) has recently given an overview of how Cancer Stem cell tumors differ from the conventional "homogenous bulk cancer
ChAT+ neurons and neurogenesis by Paez-Gonzalez et al. (Duke University)
Paez-Gonzalez et al. (Duke University) have discovered a new population of choline acetyltransferase (ChAT)+ neurons present in the rodent subventricular zone (SVZ). (1) This niche is known
Actin-Binding Proteins (ABPs) review
Actin filaments are essential for cytoskeleton functions (cell morphology, endocytosis and trafficking, contractility, motility...). In eukaryotic cells, polymerization and depolymerization of actin
Secretome biomarker identification: tech tips for selecting the most suitable tools
In a previous post, I mentioned that the secretome is becoming a very hot topic in the world of proteome analysis, and even a crucial study subject. Today, I'd like go a step further and shed some
Tumour microenvironment - inflammation and immunity
Following our series of posts on tumour microenvironment (TME) and the role of Cox-2/PGE2 signaling, I'd like today to focus on inflammation and immunity.
TME is a dynamic milieu influenced by numerous
Adipokine monitoring during Hunger and Satiety balance
Cytokines and growth factors that are produced by or have an effect on adipocytes and related peptide neuro-transmitters are central players in hunger vs. satiety balance and in eating behavior (1).