Page 8 - Scientific Library
Using CRISPR to knockout an essential gene
Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such
CRISPR genome editing: which cell line to choose?
Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.
This technology produces first a targeted break in genomic DNA, which can then be exploited to produce
Mambalgin 1 - Blocking ASIC channels in pain pathways
Acid-Sensing Ion Channels (ASICs) are neuronal voltage-insensitive cationic channels which are activated by extracellular protons. They belong to the ENaC/Deg superfamily of ion channels. Up to now 6
Focus on Actin - G-F Actin ratio measured in cells
In previous posts, I invited you to look at versatile methods to
- Visualize actin: Focus on Actin staining and visualization
- Measure actin binding: Focus on Actin – Detection of actin binding and
O-Glucuronide synthesis made easy
Efficient screening of PPAR active compounds
Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated transcription factors of the Nuclear Receptor superfamily. PPAR active compounds are of significant interest in multiple pharmaceutical
Venomous toxin ion channel modulators
Ion channels are proteins with pore-forming abilities. Their selective capabilities to let ions cross the cell membrane involves them in crucial cellular functions (controlling membrance potential, the
Cas9 mRNA optimized for genome editing
CRISPR/Cas9 is relatively simple to implement, as the researcher fully controls the experimental design of the tools, from the sgRNA sequence to the Cas9 protein.
Pharma/biotech industry outsourcing trends in Biomarker studies
A recent article by E. Langer (E. Langer, “Another In-House Operation Gets Outsourced” (2015) Pharmaceutical Technology 39 (6)) describes the growing
Ion channel over expressing cell lines for inhibitory screening
In a recent post on Venomous toxin ion channel modulators, we looked at toxin derived peptides to manipulate a wide variety of ion channels. These toxins can be used as tools to characterize