Page 4 - Scientific Library

Many recombinant proteins (rec. proteins) are now commercially available. And many of them, mainly secreted soluble proteins, are biologically active, so there shouldn't be any need to look for alternatives

A wide range of in vitro models are used in preclinical drug testing for the investigation of ADME-Tox (Absorption, Distribution, Metabolism, Excretion and oxicity) properties of New Chemical

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated transcription factors of the Nuclear Receptor superfamily. PPAR active compounds are of significant interest in multiple pharmaceutical

Isolation of highly purified untagged proteins is crucial in today's R&D programs. Up to now, recombinant protein production approaches were often based on the use of "tags" to make protein purification

Pharmaceutical, biotech and medical device industries apply restrictive quality systems in their bio-production processes. Among the numerous parameters controlled daily by scientists, I have selected

SUMO-tag gene fusion acts as both a chaperonin and as an initiator of protein folding in both prokaryotic and eukaryotic systems. Addition of this tag enables protein experts to increase the expression

Rat liver tritosomes are hepatic lysosomes that have been loaded with Tyloxapol (Triton WR 1339), a non-ionic surfactant. Tyloxapol is taken up by hepatocytes through endocytosis and is trafficked to lysosomal

In a previous post, we looked at the importance of sample preparation

Traditional methods to detect and purify poly-ubiquitinated proteins require either anti-ubiquitin antibodies or Ubiquitin Binding Associated domains (UBAs). Most of the current methods display rather

Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade data.

Transporters have become increasingly important in drug development due to the major role they play in absorption, distribution and excretion of