Page 2 - Scientific Library
SUMO-tag: boost your recombinant protein expression
SUMO-tag gene fusion acts as both a chaperonin and as an initiator of protein folding in both prokaryotic and eukaryotic systems. Addition of this tag enables protein experts to increase the expression
10 most popular antibodies in neuroscience research
Analysis of neuronal cell fates and biological activities sometimes relies on the effective staining of a protein of interest (a.k.a. marker) with a specific primary antibody validated for immuno-assays.
How sample preparation can affect your biomarker studies (II)
In a previous post, we looked at the importance of sample preparation
Proteomic profiling tools for your neurobiology studies!
First at all, a quick reminder... Cytokines play important roles in inflammation, innate immunity, angiogenesis and cell growth. They are involved in a lot of disease processes
Why is the hCMEC/D3 cell line a robust in vitro BBB model?
The Blood-Brain Barrier (BBB) affects the development of drugs for all pathologies (brain exposure to drugs, side effects...). In the early phases of drug development, there is a strong need for stable
Rolipram slows tau accumulation and AD
TUBEs: study M1 (linear) polyubiquitylated proteins
Traditional methods to detect and purify poly-ubiquitinated proteins require either anti-ubiquitin antibodies or Ubiquitin Binding Associated domains (UBAs). Most of the current methods display rather
Antibody purification troubleshooting tips
Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade data.
How to easily purify your protein with adapted magnetic beads…
…or how to make your protein attractive
Biomagnetic separation is a powerful lab tool in protein biochemistry which can be used in a wide range of applications including analysis tools
New Polyethylene Glycols (PEG) as versatile biochemical linkers
Polyethylene Glycols (aka. PEGs) are biologically inert, non-immunogenic, and hydrophilic molecules. These properties make them very attractive for research and drug discovery applications (ex. Antibody-Drug
2 new validated anti-PGP 9.5 antibodies for neuroscience research
Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase
How to choose the perfect buffer to get a pure, stabilised, functional protein
Protein biochemistry brings together a vast and varied world of methods of protein production, purification and characterization. Once you have successfully achieved the production of your protein in a