Page 2 - Scientific Library
6 ELISA test formats for protein target and biomarker discoveries
Having access to highly reproducible and fully validated ELISA tests is crucial in today's Life Science research. Although the Sandwich ELISA format is the most commonly used, other immunoassay
How do you choose the best ELISA test for your project ?
There are many, many ELISA tests out there to choose from, and making the best selection can sometimes be a bit daunting. In this post, let's take a look at the key points to keep in mind.
Human IFN ELISA test: unrivaled sensitivity and global IFN alpha detection
PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily
Cell signalling studies: Tips for selecting the best Small G Protein activation assay
Small GTP-binding proteins such as Rho and Ras GTPase family members are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology.
New one-step cGMP immunoassay with improved sensitivity
When scientists at ArborAssays invented the Direct Cyclic Nucleotide EIA immunoassay more than 20 years ago, they knew the kits would be popular. The direct nature of the format for the
Immunoassays with collagen: Tips to optimize anti-collagen antibody performance
Dr Yong Zhang, Ph.D. is a senior scientist and R&D Manager for antibody generation and assay development at Rockland Immunochemicals, Inc. who are recognised and renowned for their high quality primary
Gold standard one component TMB
Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to
Hepatic Hyaluronan Content increased by Alcohol Consumption in Patients with Liver Steatosis
In a previous post, I introduced Sekisui Xenotech's Research Biobank, which is based on rejections from liver transplantation as opposed to post-mortem tissues, thus offering a guarantee
STING: key player in drug development?
Recent articles have highlighted the role the STING cell signalling pathway may be playing in drug development, whether via its inhibition to control innate immunity and inflammation (Nature
ELISA : Quality Control and Performance Evaluation
The ELISA test (acronym of Enzyme Linked ImmunoSorbent Assay) is a test principally used In immunology to detect and/or assay a protein, antigen or antibody in a biological fluid sample . It has many practical