Scientific Library
How is Amylo-Glo a brighter, longer lasting and higher definition Amyloid plaque marker?
In a previous post I discussed 3 new unique research tools
Enhance phospho-protein identification and recovery for Western blot
Protein phosphorylation analysis is more and more used for cell signaling studies. This post-translational modification regulates the function of numerous proteins in both physiological and disease-related
Don't miss any neurotrophin!
Neurotrophins are important biomarkers in Neurobiology, including stem cell development to neural lineages. BDNF and NGF are the ones being studied in most cases. However, it has been found that neurotrophins
Get your ELISA results before lunch!
ELISAs are widely used in biomarker-related studies, especially when a high number of samples is involved, and only a few biomarkers need to be quantified. That said, incubations can be somehow cumbersome,
Ready-to-Use ELISAs to study Transcription Factors DNA binding
Gene expression is regulated by different mechanisms. One of them is the binding of
Rolipram slows tau accumulation and AD
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily