Page 4 - Scientific Library
3 easy colorimetric assays to measure ECM components
Matrix components are key R&D elements in pharmaceutical and cosmetology with tremendous impacts on tissue regeneration, skin repair,
Rat liver tritosomes in the endosome-lysosome pathway
Rat liver tritosomes are hepatic lysosomes that have been loaded with Tyloxapol (Triton WR 1339), a non-ionic surfactant. Tyloxapol is taken up by hepatocytes through endocytosis and is trafficked to lysosomal
How sample preparation can affect your biomarker studies (II)
In a previous post, we looked at the importance of sample preparation
TUBEs: study M1 (linear) polyubiquitylated proteins
Traditional methods to detect and purify poly-ubiquitinated proteins require either anti-ubiquitin antibodies or Ubiquitin Binding Associated domains (UBAs). Most of the current methods display rather
Antibody purification troubleshooting tips
Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade data.
Soluble and Insoluble Collagen assays - made easy!
The monitoring of ECM components during experimental approaches requires robust and user-friendly in vitro assays. In the past experimental methods (e.g. soluble collagen measurement) were laborious
How to easily purify your protein with adapted magnetic beads…
…or how to make your protein attractive
Biomagnetic separation is a powerful lab tool in protein biochemistry which can be used in a wide range of applications including analysis tools
New Polyethylene Glycols (PEG) as versatile biochemical linkers
Polyethylene Glycols (aka. PEGs) are biologically inert, non-immunogenic, and hydrophilic molecules. These properties make them very attractive for research and drug discovery applications (ex. Antibody-Drug
How to choose the perfect buffer to get a pure, stabilised, functional protein
Protein biochemistry brings together a vast and varied world of methods of protein production, purification and characterization. Once you have successfully achieved the production of your protein in a