Page 9 - Scientific Library
IDO and TDO - Cell based & biochemical assays
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Which roles do IDO and TDO play in Immunotherapy? Immune evasion is one of the identifying hallmarks of cancer and researchers are investigating the complex mechanisms that enable cancer cells to evade
Biomarkers associated to poor prognosis in pancreatic cancer
A recent publication by Torres et al. has found that B7-1/CD80, EG-VEGF/PK1, IL-29, NRG1-beta1/HRG1-beta1, and PD-ECGF expressions portend a poor prognosis for patients with pancreatic cancer, and these
Immunotherapy Screening – OX40:OX40L pathway
The treatment of diseases by inducing, enhancing, or surpressing an immune response is referred to as Immunotherapy. T-cell activation and inactivation requires the coordination of various co-inhibitory
SUMO-tag: boost your recombinant protein expression
SUMO-tag gene fusion acts as both a chaperonin and as an initiator of protein folding in both prokaryotic and eukaryotic systems. Addition of this tag enables protein experts to increase the expression
3 easy colorimetric assays to measure ECM components
Matrix components are key R&D elements in pharmaceutical and cosmetology with tremendous impacts on tissue regeneration, skin repair, wound healing, allergy
2 assays to assess GEF and GAP activity
In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins, such as RhoA, Rac1, Cdc42, and the proto-oncogen
Versatile measurement of protein binding to microtubules
You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.
Get your ELISA results before mid-morning coffee!
Believe it or not, some 20 years ago, performing an ELISA would take around 3 days. Now we are used to ELISAs that can be performed in one day, or even before lunch! (OK, sometimes before Spanish lunch
Proteomic profiling tools for your neurobiology studies!
First at all, a quick reminder... Cytokines play important roles in inflammation, innate immunity, angiogenesis and cell growth. They are involved in a lot of disease processes like cancer, obesity and
Tubulin polymerisation measurement - made easy!
Recently, I issued a post about a method which allows measuring microtuble binding capabilities of proteins of interest. Today, I invite you to look at methods for
Tubulin:Microtubule ratio measured in cells
Which are the best CRISPR-CAS9 strategies?
The CRISPR-CAS9 system may well have opened Pandora's box, but it is also definitely the cornucopia of genome editing.
We can do what we want in the genome: settle a mutation, correct a mutation, insert