Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bio-luminescence (Fig.
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How to monitor mitophagy in mammalian cells
Mitochondria keep cells "healthy" in their environment. In this post, we'll take a look at a new fluorescent assay for the monitoring of mitophagy, a process aimed at clearing damaged or superfluous mitochondria
Screen for inhibitors of PD-1 signalling with a complete cellular assay system
Immunotherapy represents a field in Drug Discovery which is quickly developing and leading to significant progress in treatments of a number of diseases, especially cancer. The approach is based on inducing,
LipiDye fluorescent probe for lipid droplet monitoring in live cells
LipiDye lipid droplet Green* is a new highly sensitive fluorescent probe for detecting lipid droplets in live or fixed cells. In this, let's review the characteristics of this dye which is compatible
How to activate or inhibit autophagy?
The term Autophagy was introduced by Christian de Duve during the Ciba Foundation Symposium on Lysosomes – which was held in London in February 1963. In 1974 he was honoured with
Ppc-1 small molecule: a new mitochondria uncoupling agent
Ppc-1 is a novel small molecule derived from cellular slime mould. In this post, I'd like to introduce the characteristics of this compound for your in vitro mitochondrial research.
New - Continued use immunotherapy cell line expressing recombinant PD-1
Fluorescence microscopy: how to improve your Live Cell Imaging
For more than 2 years now, the Silicon Rhodamine-like (SiR) technology has allowed the live cell imaging field with fluorescence microscopy to evolve significantly.
Fluorescent
Stauprimide inhibits c-myc transcription in cancer cells
Stauprimide is known to prime Embryonic Stem Cells (ESC) by targeting the c-Myc-activating transcription factor NME2. Its mechanism of action is linked to the inhibition of the nuclear localization
Biomolecule conjugation - how to biotinylate with reproducible results?
Biomolecule conjugation is critical to many applications such as bead-based assays, ELISA and chromatography by affinity. Unfortunately the classical methods are known to be tedious and with low