Page 3 - Scientific Library
Using CRISPR to knockout an essential gene
Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such
Cas9 mRNA optimized for genome editing
CRISPR/Cas9 is relatively simple to
How to choose the best 3D technology for your assay?
Cell culture models using 2D substrates have provided important conceptual advances in understanding the biology of cells. However, cells grown on flat 2D surfaces can differ substantially from physiological
Still using MTT or WST-1 for cell counting?
If the answer is yes, I am sure you'll be interested to learn more about WST-8 and our Cell Counting Kit-8 (CCK-8). If the answer is no, and you've already switched to WST-8, just have a look at our price
Two top ways to success with knock-out
In March 2016, Mark J Osborn et al published in Molecular Therapy a major article for genome editing (doi:10.1038/mt.2015.197), about knock-out
Simple and effective CRISPR CAS9 gene editing for primary cells
Vector-free CRISPR-CAS9 gene editing to accelerate therapeutic applications
A few years ago, Ayal Hendel et al (doi:10.1038/nbt.3290) published results revealing that chemical alterations to sgRNA
Develop robust and convenient cell based assays with Gaussian Luciferase
Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is
Easy single-cell isolation with Smart Aliquotor
Single-cell isolation allows genomic and transcriptomic analysis of one individual cell. It is also required to build a monoclonal cell line from rare cell isolation, that could be for example
Optimize your neuroprogenitor cell culture with new BDNF/GDNF Stembeads®
Neurotrophic factors are known to protect neuronal cell death in several neurodegenerative diseases, both in in vitro and in vivo. They play a key role in development, differentiation,