Page 4 - Scientific Library
3 validated tools for ubiquitination studies
Elongation of ubiquitin chains, regardless of the linkage, can form polyubiquitin fibrils to initiate the Autophagy pathway. A deficiency of the autophagy
DyLightâ„¢ will be your routine fluorescent dye
Rockland Immunochemicals have developed a suite of fluorescent dyes (DyLight™) with unique fluorescence intensity and photostability. Here, we take a look at how DyLight™ dyes have all the characteristics and potential to become your daily fluorescent dye conjugates.
#1- What makes DyLight™ dyes unique?
DyLight™ conjugates are ideal substitutes for IRDye® products as far as experimental results and budgets are concerned. For example, DyLight™ 680 and DyLight™ 800 products are an excellent alternative to Infra-Red and Near Infra-Red IRDye® products.
#2- Are DyLight™ dyes compatible with common fluorescence instrumentation?
#3- What range of applications do DyLight™ dyes cover?
DyLight™ conjugated antibodies are ideal for TrueBlot® IP / Western blotting, Multiplex imaging, Flow cytometry, IF, ELISA, High Content screening, High resolution microscopy...
We've noted that these are some of the most popular Dylight™ products:
- Anti-MOUSE IgG (H&L) (DONKEY) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039610-745-124)
- Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039610-145-121)
- Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated Pre-Adsorbed (cat. nr 039611-145-122)
- Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 680 Conjugated Pre-Adsorbed (cat. nr 039610-144-121)
Well... has this given you any ideas, would you like to learn more or try Rockland's DyLight™ fluorescent conjugated products?
Get in touch with tebu-bio's experts, or leave a message below.
Ion channel over expressing cell lines for inhibitory screening
In a recent post on Venomous toxin ion channel modulators, we looked at toxin derived peptides to manipulate a wide variety of ion channels. These toxins can be used as tools to characterize
Crosstalk between cancer and immune cells: tumor escape
A crosstalk between cancer and immune cells is established during cancers. The immune system is able to fight against tumour cells (see my previous post "Immunosurveillance: Crosstalk between cancer and
10 most popular antibodies in neuroscience research
Analysis of neuronal cell fates and biological activities sometimes relies on the effective staining of a protein of interest (a.k.a. marker) with a specific primary antibody validated for immuno-assays.
Is tumour immune evasion mediated by p53/miR-34/PDL1 interaction?
Tumour immune evasion can be mediated by a complex set of interaction involving p53, miR-34 and PDL1. This is the conclusion of a work by Cortez et al. and recently published in JCNI .
The
Why is the hCMEC/D3 cell line a robust in vitro BBB model?
The Blood-Brain Barrier (BBB) affects the development of drugs for all pathologies (brain exposure to drugs, side effects...). In the early phases of drug development, there is a strong need for stable
3 tips for IHC troubleshooting
The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results. Several factors can cause false-positive or false-negative
Post-Translational Modifications (PTM) of Tubulin
In this post, I'd like to take a look at the current understanding of tubulin PTMs, that include tyrosination/detyrosination, Δ2-tubulin formation, acetylation, phosphorylation, ubiquitination, glutamylation,
2 new validated anti-PGP 9.5 antibodies for neuroscience research
Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase
New Zika virus monoclonal antibodies
Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals, 100% validated
New effective anti-Human IFN neutralizing antibody
PBL Assay Sciences offer a new monoclonal antibody to neutralize Human Interferon (IFN)