Page 3 - Scientific Library

PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha

Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

Interestingly, the experimental design is simple and is based on 3 steps:

1. Plate
2. Treat and fix your cells of interest (adherent or in suspension)
3. Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)

These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily

Small GTP-binding proteins such as Rho and Ras GTPase family members are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology.

As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras

When scientists at ArborAssays invented the Direct Cyclic Nucleotide EIA immunoassay more than 20 years ago, they knew the kits would be popular. The direct nature of the format for the

Dr Yong Zhang, Ph.D. is a senior scientist and R&D Manager for antibody generation and assay development at Rockland Immunochemicals, Inc. who are recognised and renowned for their high quality primary

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

RNA-based CRISPR-CAS9 gene editing is a vector-free approach that is required for therapeutic perspectives. However, from a practical point of view, what appears as a major challenge to engineer

Messenger RNA can be used for protein expression into the cells. The delivery of mRNA is the delivery of a new function that can activate a biological process. In the particular case of the CAS9 mRNA,

Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to

The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in