Page 6 - Scientific Library
microRNAs: Target detection
Leading on from my previous posts (see below) exploring microRNA detection, overexpression and inhibition, this time, let's spend a moment on target detection.
miTarget databases
Antibody pairs - the rebirth of a classic
Antibody pairs have been used for years to detect biomarkers in biological samples. They are a convenient and price-wise alternative to ready-to-use ELISAs, especially when they are used on a routine basis
Focus on the Hypoxia Regulation Mechanism
The cellular and physiological effects resulting from hypoxia-dependent networks have been clearly shown to impact a number of human pathological states, including ischemic disease, diabetes, pulmonary
Tumour microenvironment - glioblastoma
Factors released by tumour cells can have an impact on the outcome of cancer in a patient, and they can be used as diagnostic or prognostic biomarkers. A deeper study on the tumour microenviroment (TME)
Gateway® cloning technology: Is it as easy as they say?
Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.
Basically, the technology allows you to start
6 Tips for a successful ELISA
ELISA are immunoassays widely used in biomarker detection and validation. They have been used in research and clinical settings for more than 40 years, and they allow to quantify, in a simple way, thousands
3 Reasons to use fresh TLR9 agonists
As part of the innate immune response, Toll-like receptor 9 recognizes unmethylated CpG nucleotide sequences, which are very common in viruses and prokaryotes and more rare in vetebrates. Scientists routinely
Sample interference in ELISA tests
A previous post explained how samples obtained from patients treated with mAbs can show interference with WB & ELISA methods. More rarely, we can also find other interference effects
Hyaluronic acid - size matters!
Hyaluronic acid (HA), or hyaluronan, is an ubiquitous, very high molecular mass polysaccharide that has applications
What's new in qPCR?
We know molecular biologists spent a lot of time setting up their qPCR protocols and aren't about to change the way they do things. We also know they are technically savvy and like keeping up with the
Alternatives to the "mega-brands" of molecular biology
If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?
Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,
Genome Editing in Stem Cells: outsource or do-it-yourself?
Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing