Page 2 - Scientific Library

In the field of Drug Development, 3D cell culture models such as spheroids or organoids currently appear as one of the best solutions to obtain accurate and physiological in vitro

Focus Biomolecules produces a broad range of bioactive lipid mediators as well as many small molecule inhibitors of numerous lipid metabolic pathways for use as cellular

Recombinant CYPs are commonly used in ADME-Tox studies as in vitro tools, mostly for reaction phenotyping, enzyme inhibition screening, clearance and metabolite ID.

"Research

Over the years, Sekisui XenoTech has built up a strong expertise in preparing subcellular fractions for ADME applications.

There is

Sekisui XenoTech now offers human liver tissue microarrays (TMA) and a microsomal pool for studying and developing new treatments for fatty liver disease (FLD). The

In a few forthcoming posts, I'd like to share several short articles published by Dr Chris Bohl of Sekisui Xenotech, a technical expert in the field of ADME-Tox tools and applications. I feel they may

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

Interestingly, the experimental design is simple and is based on 3 steps:

1. Plate
2. Treat and fix your cells of interest (adherent or in suspension)
3. Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)

These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily

Following up on my series of posts based on Dr Chris Bohl's work at Sekisui Xenotech, in this post I invite you to take a look at the work he has published, together with Dr Christopher Seib, Maciej Czerwinski,

Pooled human hepatocytes are a preferred test system in many drug discovery and development applications requiring intact cellular systems for in vitro testing. Intact hepatocytes contain the

NADPH is a critical cofactor supporting numerous biochemical reactions. In ADME-Tox studies, NAD(P)H regeneration is strongly recommended when using drug metabolizing enzymes (ex. Cytochrome

Membrane transporters can have clinically relevant effects on the pharmacokinetics and pharmacodynamics of a drug in various organs and tissues by controlling its absorption, distribution and elimination.

I am following up on my series of posts based on the work done by Chris Bohl and collaborators at Sekisui Xenotech. Here, he looks at the effect of cryopreservation on pooled hepatocytes metabolic activities,