Page 10 - Scientific Library
Blood as a cells highway
For years, we already
Using CRISPR to knockout an essential gene
Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such
CRISPR genome editing: which cell line to choose?
Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.
This technology produces first a targeted break in genomic DNA, which can then be exploited
9 pathway-specific screening assays in Immunotherapy
8 criteria for selecting your ELISA kits
Biomarkers specialists are often asked to select an ELISA kit for researchers: with thousands of ELISA references available on the market, the choice can be tricky regarding proteins for which several
Interferons - the first line of defense against viral infections
Interferons (IFNs) are low molecular weight proteins that belong to the class of glycoproteins known as cytokines. IFNs are part of the non-specific immune system and are an important first line of defense
How sample preparation can affect your biomarker studies (I)
In previous posts, we have discussed about whether samples should be pooled, how much (and if) they should be diluted, and how biologicals can affect the
Cas9 mRNA optimized for genome editing
CRISPR/Cas9 is relatively simple to
Pharma/biotech industry outsourcing trends in Biomarker studies
A recent article by E. Langer
How to easily obtain a highly purified untagged recombinant protein
Isolation of highly purified untagged proteins is crucial in today's R&D programs. Up to now, recombinant protein production approaches were often based on the use of "tags" to make protein purification
STAT3 signaling effects on IL-15 derived dendritic cells
The role of STAT3 signaling in the activity of dendritic cells (DC) has been recently revealed in a publication by Okada et al.
Secretome profiling - now quantitative!
For secretome biomarker profiling, several solutions, including arrays allowing to screen up to 1,000 factors, have been available for some time now. These profiling solutions, however,