Scientific Library

We have been closely following the interesting case of the discontinuation of tobacco acid pyrophosphatase (TAP) and the efforts of the world's RNA biologists to find a suitable alternative. Our previous

For about 5 years now, there has been a renewed interest for mRNA molecules and their numerous applications. Mature mRNAs can be transfected into cells (ex. in mammalian cells) for various purposes

Three papers on the role of the TGF-beta pathway in different cancers have recently been published.

It was already known that this pathway is involved in processes such as cell growth, cell differentiation,

The Wnt cell signalling pathway is a high-potential therapeutical target in cancer research. It's deeply linked to cellular fate through modulating cell proliferation, mobility, interaction, and polarity.

In drug discovery screening campaigns as well as in fundamental research activities, cellular parameters have to be measured and monitored in living cells regularly. Often fluorescent dyes are used to

In a recent publication, researchers from the University of Miami Miller School of Medicine (USA) describe that Serotonin released by human beta cell inhibits glucagon secretion by alpha cells. They demonstrated

Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is Firefly

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

Non-invasive manipulations of intracellular processes have recently appeared as an emerging research field. Experts in the development of biosensors for live cell discovery, Montana Molecular provide an

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of