Page 3 - Scientific Library
Obtain clear Western Blot and Immunoprecipitation results
Generating high-quality Immunoprecipitation & Western Blot (IP & WB) data has never been easier. See how you can easily achieve this in our Tips & Tricks section to get clean WB-IP.
Breast Cancer - Popular validated primary antibodies for research and biomarker discovery
Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we'll review the main biomarkers involved in this disease and introduce the
ELISA test to confirm elimination of Host Cell Proteins (HCP) from bioproductions
Expression systems - removing their contaminants
CHO, E. Coli and Pichia are useful expression systems for drug production. This can be for proteins, enzymes or antibody productions. Research and
6 ELISA test formats for protein target and biomarker discoveries
Having access to highly reproducible and fully validated ELISA tests is crucial in today's Life Science research. Although the Sandwich ELISA format is the most commonly used, other immunoassay
How do you choose the best ELISA test for your project ?
There are many, many ELISA tests out there to choose from, and making the best selection can sometimes be a bit daunting. In this post, let's take a look at the key points to keep in mind.
Human IFN ELISA test: unrivaled sensitivity and global IFN alpha detection
PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha
New - 4 Unique IFN Neutralizing reagents
PBL Assay Science, the specialists of Interferon related quality reagents, have just released 4 new unique products to be used in neutralization assays. I am pleased to introduce them in this post, in
Where to find antigens and antibodies for Enterovirus-specific Assay development
Enteroviruses are common human viruses causing rather mild symptoms. However many different species and serotypes (incl. Echoviruses, Polioviruses,
Find high performing monoclonal antibodies for your Renin-specific ELISA tests
Released by the kidneys, Renin is an enzyme which belongs to the Renin-Angiotensin system (RAS) and is involved in blood pressure homeostasis.
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily
Cell signalling studies: Tips for selecting the best Small G Protein activation assay
Small GTP-binding proteins such as Rho and Ras GTPase family members are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology.