It is widely accepted that data obtained from primary cells is not only desired, but, most relevant when trying to study physiological interactions. Experimentation in primary cells allows a deeper and

Successful culture of primary cells requires obtaining a maximum yield of functionally viable single cells from whole tissues. The primary factors that affect successful dissociation of whole tissue have

Cancer research is increasingly focusing on the tumour microenvironment (TME). Several studies have shown that tumours depend on external signals for maintenance and expansion. It is therefore needed

Antibody pairs have been used for years to detect biomarkers in biological samples. They are a convenient and price-wise alternative to ready-to-use ELISAs, especially when they are used on a routine basis

The influenza season in Europe is over... until next year. Influenza remains one of the most important public healthcare problems, especially in risk groups.

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocorticotropic hormone. Corticosterone is a major indicator of stress in non-human mammals.

RIPA buffer is one of the most useful protein extraction buffers and maintains most native structures of proteins. Nevertheless, RIPA buffer is not convenient enough to extract membrane proteins and membrane-associated

Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals,

Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part

Tissue culture for Primary cell isolation are often jeopardized by the outgrowth of contaminating fibroblasts. As fibroblasts usually grow at a much faster rate compared to the target cell population,

Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System