Page 2 - Scientific Library
What's new in qPCR?
We know molecular biologists spent a lot of time setting up their qPCR protocols and aren't about to change the way they do things. We also know they are technically savvy and like keeping up
Next generation of lentiviral particles
Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and
Exosomes as a trigger for signaling
Extracellular vesicles in general, or exosomes in particular, are becoming a hot topic for research, especially in cancer. They seem to have different roles in tumour progression or can be used as
Adenovirus: weapons of mass transduction for gene delivery
Adenovirus (AV) are non-enveloped and non-integrative icosaedral viruses. Recombinant AV (genetically modified with respect to the wild-type in order to lose their capacity of replication) are widely used
Alternatives to the "mega-brands" of molecular biology
If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?
Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,
Kit-Free Site Directed Mutagenesis Protocol
Molecular biologists are familiar with the QuikChange® Site-Directed Mutagenesis Kit that allows rapid intoduction of a point mutant into a plasmid/vector/mammalian expression construct. Briefly, the protocol
How to obtain reliable DNA and RNA quantitation
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in vitro
ORF expressing Lentiviral system
ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell
Gene knock-out in hard-to-transfect cells
Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of
What is the connection between COVID-19 severity and gene expression in immune cells?
The immune system, responsible for fighting off infections and diseases, refers to multiple biological processes that have different functions and contributions to the immune system and its response to
What diseases are linked to Microbiota disturbance?
The microbiota is the set of microbial organisms in a community, generally used in reference to an animal host. The digestive microbiota is the most common.
What we call a microbiome is the set of microorganisms
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