Page 3 - Scientific Library
Crosstalk between cancer and immune cells: tumor escape
A crosstalk between cancer and immune cells is established during cancers. The immune system is able to fight against tumour cells (see my previous post "Immunosurveillance: Crosstalk between cancer
3 easy colorimetric assays to measure ECM components
Matrix components are key R&D elements in pharmaceutical and cosmetology with tremendous impacts on tissue regeneration, skin repair, wound healing, allergy
2 assays to assess GEF and GAP activity
In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins, such as RhoA, Rac1, Cdc42, and the proto-oncogen
Histone Deacetylases (HDACs) and Alzheimer
In a recent post, I concentrated on the role of HDACs in cancer: Histone Deacetylases (HDACs) and Cancer and mentioned that some HDACs are overexpressed and show very high activities in a number of tumours.
Versatile measurement of protein binding to microtubules
You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.
Is tumour immune evasion mediated by p53/miR-34/PDL1 interaction?
Tumour immune evasion can be mediated by a complex set of interaction involving p53, miR-34 and PDL1. This is the conclusion of a work by Cortez et al. and recently published in JCNI .
The involvement
Tubulin polymerisation measurement - made easy!
Recently, I issued a post about a method which allows measuring microtuble binding capabilities of proteins of interest. Today, I invite you to look at methods for
Tubulin:Microtubule ratio measured in cells
Genetically encoded fluorescent biosensors for live cell discovery
In drug discovery screening campaigns as well as in fundamental research activities, cellular parameters have to be measured and monitored in living cells regularly. Often fluorescent dyes are used to