Page 4 - Scientific Library

To dilute or not to dilute biological samples? And if yes, how much? That's the question!

Following our post on whether samples should be pooled or not when performing studies for

Back to dilution of samples. After our post on whether samples should be diluted or not, let's discuss today about how much they should be diluted.

Case # 1 - ELISA or simplex

ELISAs are widely used in biomarker-related studies, especially when a high number of samples is involved, and only a few biomarkers need to be quantified. That said, incubations can be somehow cumbersome,

Molecular biologists are familiar with the QuikChange® Site-Directed Mutagenesis Kit that allows rapid intoduction of a point mutant into a plasmid/vector/mammalian expression construct. Briefly, the protocol

Hyaluronic Acid (HA) is important in many biological processes such as wound repair, tissue hydration and inflammation. HA is also a potential biomarker for diseases such as osteoarthritis, liver cirrhosis

Gene expression is regulated by different mechanisms. One of them is the binding of

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocorticotropic hormone. Corticosterone is a major indicator of stress in non-human mammals.

The neuropeptides, Oxytocin and Vasopressin, were isolated and synthesized by Vincent du Vigneaud at Cornell Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955. Oxytocin

The determination of serum blood urea nitrogen (BUN) is currently the most widely used screening test for the evaluation of kidney function. The test is frequently run along with the serum creatinine test,

PGE₂

Classically, it was known that CD4⁺ T cells, upon activation and expansion, develop into different T helper cell subsets with different cytokine profiles and distinct effector functions to effect

Immunoediting of cytokine signals provides another area of pathways which tumours utilise to evade and potentially escape from immunological targeting. This post aims at shedding some light into the Tumour