Scientific Library
microRNAs: Keys to open cell biology’s secret chamber
As cell biologist, when working in labs I have in the past witnessed several “X-files” in a flask of cells along my way: all cells of an established cell line are supposed to have the same genetic background
microRNAs: Finding the effector - miRNA detection
Following on from my previous post in which I started to introduce a series of techniques to investigate the impact of miRNA expression on cell fate,
microRNAs: Push and observe - miRNA overexpression
In this third post of the miRNA-related series, let's take a look at miRNA overexpression.
miRNA mimics
Short RNAs are very prone to enzymatic degradation. Novel chemical technologies now
microRNAs: Inhibiting the inhibitors - Antagomirs
Following on in our series on microRNAs (miRNA - Keys to open cell biology’s secret chamber)
microRNAs: Target detection
Leading on from my previous posts (see below) exploring microRNA detection, overexpression and inhibition, this time, let's spend a moment on target detection.
miTarget databases
Gateway® cloning technology: Is it as easy as they say?
Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.
Basically, the technology allows you to start
Genome Editing in Stem Cells: outsource or do-it-yourself?
Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing
Using CRISPR to knockout an essential gene
Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such
Develop robust and convenient cell based assays with Gaussian Luciferase
Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is
Luciferase promoter reporter clones
Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient
shRNA set with improved performances
Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the