Kupffer primary cells are macrophages endogenous to the liver which have the ability to modulate hepatic inflammation and injury associated with various pathophysiologies and toxicities. Pro-inflammatory

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

Involved in many physiological processes (apoptosis, aging, cell signalling..) and numerous diseases (cardiovascular disorders, to cancer and neuro-degenerative diseases…), Mitochondria have become more

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of

All human and living beings have mitochondria. These are tiny cellular organisms present in nearly every cell of an individual. They are located more precisely inside the cells, in their nucleus. The main

Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration

In their latest publication in Nature, Dr. Moiseeva et.al., studied cellular senescence in skeletal muscle, carrying

Mitochondrial Dynamics and Mitochondria-Lysosome Contacts in Neurogenetic Diseases

In an article published in frontiers in Neuroscience, with the aim to study cellular phenotypes in fibroblasts from

Understanding cellular senescence and oxygen consumption rate

A recent paper published in Science reported a 50% reduction in mortality and improved immune response in old mice infected with SARS-CoV-2

Relationship between antitumor effects and active oxygen

ROS (Reactive oxygen species) are highly reactive oxygen species mainly generated during the ATP synthesis process in mitochondria. While it is