Scientific Library
Enhance phospho-protein identification and recovery for Western blot
Protein phosphorylation analysis is more and more used for cell signaling studies. This post-translational modification regulates the function of numerous proteins in both physiological and disease-related
Vive la différence! To pool or not to pool...
In a previous post on whether samples should be pooled or not for proteomic profiling, we discussed this approach, which can be quite cost-wise, while still allowing to see the main biomarkers
How sample preparation can affect your biomarker studies (I)
In previous posts, we have discussed about whether samples should be pooled, how much (and if) they should be diluted, and how biologicals can affect the
Following your proteins - anti-tag antibodies (I)
When producing recombinant proteins, one can follow the purification process either by antibodies detecting the target protein specifically, or by adding a tag to it, thus using antibodies detecting not
New - Continued use immunotherapy cell line expressing recombinant PD-1
Quantify ExtraCellular Matrix components - 5 popular kits you need to know
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily
AIM Biochips - 3D cell culture platform for Immunotherapy and T Cell therapy studies
As mentioned in a previous post the DAX Biochips from AIM Biotech are an innovative and versatile microfluidic platform, allowing researchers to more easily develop 3D cell culture
Three new pseudoviruses for SARS-CoV-2 studies
To better understand the mechanism of SARS-CoV-2 cell entry, BPS Bioscience have developed 3 new pseudotyped lentiviruses, allowing