Page 3 - Scientific Library
Using CRISPR to knockout an essential gene
Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such
More data with chemiluminescence imaging platform
Depending on the phase where your biomarker-related research program is,
8 criteria for selecting your ELISA kits
Biomarkers specialists are often asked to select an ELISA kit for researchers: with thousands of ELISA references available on the market, the choice can be tricky regarding proteins for which several
Cytokine signature in airway inflammation with arrays
Chronic Mucosal Inflammation is the hallmark of common airway diseases (ex. Allergic Rhinitis and asthma). Lipoxin B4(LXB4) is an endogenous mucosal protective mediator decreasing
Cas9 mRNA optimized for genome editing
CRISPR/Cas9 is relatively simple to
Pharma/biotech industry outsourcing trends in Biomarker studies
A recent article by E. Langer
STAT3 signaling effects on IL-15 derived dendritic cells
The role of STAT3 signaling in the activity of dendritic cells (DC) has been recently revealed in a publication by Okada et al.
Two top ways to success with knock-out
In March 2016, Mark J Osborn et al published in Molecular Therapy a major article for genome editing (doi:10.1038/mt.2015.197), about knock-out
Simple and effective CRISPR CAS9 gene editing for primary cells
Vector-free CRISPR-CAS9 gene editing to accelerate therapeutic applications
A few years ago, Ayal Hendel et al (doi:10.1038/nbt.3290) published results revealing that chemical alterations to sgRNA
Develop robust and convenient cell based assays with Gaussian Luciferase
Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is
Evolutions in Molecular Cloning to save time and make life easy
As with this painted representation of (surrealist!) DNA, to a certain extent, molecular cloning is an art.
Unfortunately for newcomers, or fortunately for experts, it's required for many downstream