Page 3 - Scientific Library
Simple and effective CRISPR CAS9 gene editing for primary cells
Vector-free CRISPR-CAS9 gene editing to accelerate therapeutic applications
A few years ago, Ayal Hendel et al (doi:10.1038/nbt.3290) published results revealing that chemical alterations to sgRNA
Develop robust and convenient cell based assays with Gaussian Luciferase
Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is
Easy single-cell isolation with Smart Aliquotor
Single-cell isolation allows genomic and transcriptomic analysis of one individual cell. It is also required to build a monoclonal cell line from rare cell isolation, that could be for example
Optimize your neuroprogenitor cell culture with new BDNF/GDNF Stembeads®
Neurotrophic factors are known to protect neuronal cell death in several neurodegenerative diseases, both in in vitro and in vivo. They play a key role in development, differentiation,
Luciferase promoter reporter clones
Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient
How to obtain reliable DNA and RNA quantitation
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in
How to get rid of fibroblastic contaminating cells?
Tissue culture for Primary cell isolation are often jeopardized by the outgrowth of contaminating fibroblasts. As fibroblasts usually grow at a much faster rate compared to the target
Lympholyte®: The ideal tool for simple and viable lymphocytes isolation
High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE®
shRNA set with improved performances
Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the