Page 6 - Scientific Library
Adipokines in obesity and glucose homeostasis
Obesity is the abnormal increase in adipose tissue mass. This phenomenon increases the likelyhood of a number of diseases such as heart disease, high blood pressure, type 2 diabetes, obstructive sleep
Antibody pairs - the rebirth of a classic
Antibody pairs have been used for years to detect biomarkers in biological samples. They are a convenient and price-wise alternative to ready-to-use ELISAs, especially when they are used on a routine basis
Tips for Optimizing Cell Culture Conditions with Hydrogels
In a previous post on Cellular Matrices for Translational Research, I introduced HyStem hydrogels. In order to engineer
Tumour microenvironment - glioblastoma
Factors released by tumour cells can have an impact on the outcome of cancer in a patient, and they can be used as diagnostic or prognostic biomarkers. A deeper study on the tumour microenviroment (TME)
6 Tips for a successful ELISA
ELISA are immunoassays widely used in biomarker detection and validation. They have been used in research and clinical settings for more than 40 years, and they allow to quantify, in a simple way, thousands
Sample interference in ELISA tests
A previous post explained how samples obtained from patients treated with mAbs can show interference with WB & ELISA methods. More rarely, we can also find other interference effects
Hyaluronic acid - size matters!
Hyaluronic acid (HA), or hyaluronan, is an ubiquitous, very high molecular mass polysaccharide that has applications
Diluting samples for proteomics – biomarker profiling case studies (I)
To dilute or not to dilute biological samples? And if yes, how much? That's the question!
Following our post on whether samples should be pooled or not when performing studies for
Biomarker profiling: Diluting samples case studies (II)
Back to dilution of samples. After our post on whether samples should be diluted or not, let's discuss today about how much they should be diluted.
Case # 1 - ELISA or simplex
HD-LCI video of HUVEC Transfection with Cytofect Kit
See human cells glow with green fluorescent protein in this time-lapse video. These images were captured over 20 hours via Lumascope 620.
[youtube https://www.youtube.com/watch?v=k5esUe_rrr4]
Hepatic Cellular Models
A wide range of in vitro models are used in preclinical drug testing for the investigation of ADME-Tox (Absorption, Distribution, Metabolism, Excretion and oxicity) properties of New Chemical