Page 4 - Scientific Library
FAQs on feeder cells for optimal human iPSC culture
Human Newborn Foreskin Fibroblasts (NuFF cells) are genuine feeder cells for Stem cell research. Mitotically inactive NuFF cells (by irradiation or Mitomycin C chemical treatment) can efficiently maintain
Human oligodendrocyte MO3.13 cells & Globoid cell leukodystrophy
In this paper (Role of extracellular calcium and mitochondrial oxygen species in psychosine-induced oligodendrocyte cell death), Voccoli et al. used Human oligodendrocyte MO3.13 cells to study Globoid
MDR1 activity enhancement reduces senescence markers
During the last International Federation of Societies of Cosmetic Chemists (IFSCC) congress (Paris, Oct. 2014), Hajem N. et al (ALES Groupe) have shown that by enhancing MDR activity of fibroblasts
AAV - advantages for Gene delivery in vivo
Adenoassociated viruses (AAV) are small (20-30 nm), ssDNA, non-enveloped and replication-defective viruses that infect human and other primate species, but which, up to now, have not been reported as being
Predicting CYP induction in stable cell lines
Assessing gene transcription as an endpoint for determining induction of drug metabolizing enzymes and transporters has become the “method of choice” in the FDA’s latest draft Guidance for Industry drug
Next generation of lentiviral particles
Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and
Exosomes as a trigger for signaling
Extracellular vesicles in general, or exosomes in particular, are becoming a hot topic for research, especially in cancer. They seem to have different roles in tumour progression or can be used as
Adenovirus: weapons of mass transduction for gene delivery
Adenovirus (AV) are non-enveloped and non-integrative icosaedral viruses. Recombinant AV (genetically modified with respect to the wild-type in order to lose their capacity of replication) are widely used
PBMCs & drug development
Use of human samples including peripheral blood mononuclear cells (PBMCs) in drug discovery is critical for increasing the chances of success for a small molecule screen hit. A review based on recent
Cellular models for studying the human urogenital system
The urogenital system is the organ system of the reproductive organs and the urinary system. These are grouped together because of their proximity to each other, their common embryological origin and the
Cellular models to study the cardiac System (part I)
In this post, I'd like to introduce human aortic, brachiocephalic, carotid artery and coronary artery cells isolated by Cell Applications Inc. In a future post, I'll be highlighting human internal
Cellular models to study the Cardiac System (part II)
In a previous post, I introduced several models to study the cardiac human system. This first post introduced human aortic, brachiocephalic, carotid artery and coronary artery cells isolated by Cell Applications