Page 4 - Scientific Library
Bioactive neuronal lipid mediators: a focus on Synaptamide
Focus Biomolecules produces a broad range of bioactive lipid mediators as well as many small molecule inhibitors of numerous lipid metabolic pathways for use as cellular
GPR17 and Remyelination: activators and antagonists
The absence of mature oligodendrocytes for the repair of demyelination lesions is a critical factor in the pathology of Multiple Sclerosis (MS), and other debilitating CNS disorders
Human IFN ELISA test: unrivaled sensitivity and global IFN alpha detection
PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily
Cell signalling studies: Tips for selecting the best Small G Protein activation assay
Small GTP-binding proteins such as Rho and Ras GTPase family members are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology.
Improve MS-based biomarker determination with immunocapture
During the past decade, mass spectrometry, notably LC/MS, has become a major approach to identify and quantify proteins in patient samples. It includes analysis before treatments to find the proper biomarkers
Powerful and reliable solutions to decipher Actin Dynamics
As is well known, the actin cytoskeleton is a highly dynamic structure, involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion,
New one-step cGMP immunoassay with improved sensitivity
When scientists at ArborAssays invented the Direct Cyclic Nucleotide EIA immunoassay more than 20 years ago, they knew the kits would be popular. The direct nature of the format for the
Immunoassays with collagen: Tips to optimize anti-collagen antibody performance
Dr Yong Zhang, Ph.D. is a senior scientist and R&D Manager for antibody generation and assay development at Rockland Immunochemicals, Inc. who are recognised and renowned for their high quality primary
Discover the world's first Intracellular Polyamine Imaging Reagent
With their polycationic properties, polyamine organic compounds are involved in various biological functions (DNA-RNA interaction, gene expression...), and can also be considered as cancer marker.
Gold standard one component TMB
Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to