Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bio-luminescence (Fig.
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LipiDye fluorescent probe for lipid droplet monitoring in live cells
LipiDye lipid droplet Green* is a new highly sensitive fluorescent probe for detecting lipid droplets in live or fixed cells. In this, let's review the characteristics of this dye which is compatible
How to activate or inhibit autophagy?
The term Autophagy was introduced by Christian de Duve during the Ciba Foundation Symposium on Lysosomes – which was held in London in February 1963. In 1974 he was honoured with
Ppc-1 small molecule: a new mitochondria uncoupling agent
Ppc-1 is a novel small molecule derived from cellular slime mould. In this post, I'd like to introduce the characteristics of this compound for your in vitro mitochondrial research.
Fluorescence microscopy: how to improve your Live Cell Imaging
For more than 2 years now, the Silicon Rhodamine-like (SiR) technology has allowed the live cell imaging field with fluorescence microscopy to evolve significantly.
Fluorescent
Stauprimide inhibits c-myc transcription in cancer cells
Stauprimide is known to prime Embryonic Stem Cells (ESC) by targeting the c-Myc-activating transcription factor NME2. Its mechanism of action is linked to the inhibition of the nuclear localization
New Reporter cell lines for Drug discovery and Immunotherapy checkpoint research
In the last few decades, in the field of Drug Discovery, Immunotherapy has gained more and more importance for the treatment of various diseases and in particular for certain types of
Biomolecule conjugation - how to biotinylate with reproducible results?
Biomolecule conjugation is critical to many applications such as bead-based assays, ELISA and chromatography by affinity. Unfortunately the classical methods are known to be tedious and with low
How can you improve your reporter gene assays?
Gene reporter assays are widely used in research and Drug Discovery. The development of such cell lines is often time-consuming and costly, sometimes upwards of 5-20k € per cell line.
Immunotherapy research: Easily build your own reporter cell line
As discussed previously in our recent Drug Discovery post, Immunotherapy is one of the central research fields for various disease treatments and also certain cancers. A major regulator
Bioactive neuronal lipid mediators: a focus on Synaptamide
Focus Biomolecules produces a broad range of bioactive lipid mediators as well as many small molecule inhibitors of numerous lipid metabolic pathways for use as cellular