Page 5 - Scientific Library
Next generation of lentiviral particles
Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and
Easy and sensitive methods to measure antioxidative potential
Free radicals and reactive oxygen species (ROS) are highly reactive molecules that are generated by normal cellular processes, environmental stress, and UV irradiation. ROS react with cellular components,
Exosomes as a trigger for signaling
Extracellular vesicles in general, or exosomes in particular, are becoming a hot topic for research, especially in cancer. They seem to have different roles in tumour progression or can
Adenovirus: weapons of mass transduction for gene delivery
Adenovirus (AV) are non-enveloped and non-integrative icosaedral viruses. Recombinant AV (genetically modified with respect to the wild-type in order to lose their capacity of replication) are widely used
How to measure Glycosaminoglycans and Proteoglycans?
Glycosaminoglycans (or mucopolysaccharides) are long un-branched polysaccherides which consist of disaccharide repeats. The repeats usually (with the exception of Keratan sulfate) consist of on
Caspases as pharmaceutical targets - screening for inhibitors?
Caspases (cysteine-dependent aspartate-directed proteases) belong to the family of cysteine proteases and are involved in networks controlling cell death (apoptosis and necrosis) and
Differentiate between K48- and K63-specific Ubiquitination
Ubiquitination is a common post translational protein modification that occurs through an isopeptide linkage between the C-terminus of ubiquitin and the ε-amino group of a lysine residue on the target
A simple way to measure Elastin
Recently I reported about methods to measure:
- Collagen - Sircol for soluble and insoluble collagen assays
- Glycosaminoglycans - How to measure Glycosaminoglycans
9 pathway-specific screening assays in Immunotherapy
Focus on Actin - G-F Actin ratio measured in cells
In previous posts, I invited you to look at versatile methods to
- Visualize actin: Focus on Actin staining and visualization
- Measure