Page 3 - Scientific Library
Evolutions in Molecular Cloning to save time and make life easy
As with this painted representation of (surrealist!) DNA, to a certain extent, molecular cloning is an art.
Unfortunately for newcomers, or fortunately for experts, it's required for many downstream
How is inflammation quantification evolving?
Inflammation is involved in many fields including cosmetology, drug discovery (including RNA-based vaccines), and diagnostic index development, as well as being linked to almost every disease such as HIV,
Establish if a treatment modulates immune response, even in a conflicting background
In a recently published study, researchers sought to elucidate if 2 treatments modulated immune response or not, despite the available evidence being conflicting. The method that made the
How a multiplex ELISA helps development of rapid diagnostic tests
One of the challenges in R&D is to find robust technologies to develop diagnostic tests. Notably, it could be within a context of asymptomatic infections for which reliable detection of positive specimens
Luciferase promoter reporter clones
Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient
Achieve success with RNA-based CRISPR-CAS9 knock-out
RNA-based CRISPR-CAS9 gene editing is a vector-free approach that is required for therapeutic perspectives. However, from a practical point of view, what appears as a major challenge to engineer
How to produce high yield capped mRNA
Messenger RNA can be used for protein expression into the cells. The delivery of mRNA is the delivery of a new function that can activate a biological process. In the particular case of the CAS9 mRNA,
How to obtain reliable DNA and RNA quantitation
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in
Study 10 cytokines and more for the price and simplicity of a single ELISA
I just want to briefly outline in this post the power of antibody microarray when it comes to time and cost saving as well as versatility. Whether you want to do multiple profiling or
Want to know more about your Biologics?
In new drug development pipelines, there is an increasing trend to switch from "small" to "large" molecules (also known as biologics). While the FDA guidance was quite specific with their recommendations
New - you know CRISPR, now it's Prime Editing
This month, a new leap forward in gene editing has been published in Nature under the title 'Search-and-replace genome editing without double-strand breaks or donor DNA'. DOI: 10.1038/s41586-019-1711-4.
How to monitor inflammatory cytokines in anti-COVID drug discovery
The close relation between Severe Acute Respiratory Syndrome (SARS), unpaired adaptive immune responses and overexpression of inflammatory cytokines has been described more than a decade